Reagents and Labware
THE® Anti-His mAb - Description
Monoclonal antibodies specific to polyhistidine can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. However, since 6xHis-tag is poorly immunogenic, it needs to be conjugated to KLH or some other carrier as an immunogen. After hundreds of selection cycles, researchers at GenScript successfully isolated an antibody against His-tag. This antibody (subtype IgG1) has very high affinity. Tests performed at GenScript show that the antibody can also recognize 4xHis- and 5xHis-tags. This means that even if t he 6xHis-tag is only partially exposed, it will still be recognized and bound by this antibody. THE® Anti-His mAb is produced from mice ascites and purified by protein G affinity column. This antibody recognizes native as well as denatured forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The product reacts with fusion proteins expressed in bacteria, insect cells, and mammalian cells.
Culture Medium >> enquire
Consumables - Various sizes of Tips, Thin wall PCR tubes, 96/384 Thin wall PCR paltes >> enquire
THE® Anti-His mAb
recognizes polyhistidine tags placed at N-terminal, C-terminal, and internal regions of fusion proteins. THE® Anti-His mAb can be used in Western blot analyses, Dot blot analyses, ELISA, immunofluorescent staining, and flow cytometry of cultured cells.
Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 1:10,000-1:100,000
Western blot: 1:5,000-1:10,000
Immunoprecipitation (IP): 1:1, 000
Immunofluorescent staining: 1:1,000
FAC assay/Flow cytometry: 1:1,000
Other Applications: user-optimized